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human lung cancer  (ATCC)


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    ATCC human lung cancer
    Human Lung Cancer, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lung+cancer/TCMK-1/pm42298006-50-17-37
    Average 96 stars, based on 208 article reviews
    human lung cancer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Multiple Displacement Amplification:

    Article Title: Design, Synthesis, Analysis, and Cytotoxicity of Novel Heteroaryl Derivatives of Dipyridothiazines
    Article Snippet: .. The cell lines used in this study—human breast adenocarcinoma (MDA-MB-231), human lung cancer (A-549), and immortal HaCaT keratinocytes—were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA), ensuring their verified origin and adherence to reference characteristics. .. Each line was maintained according to ATCC-recommended specifications for its cell type.

    Article Title: Compounds and methods for the treatment of PKR-associated diseases
    Article Snippet: .. Cell lines: Human lung cancer (A549, H1299, H1792, H460, H322, H292, and H226), breast cancer (MDA-MB-231 and MCF-7), and colon cancer (LOVO) cell lines were obtained from the ATCC (Manassas, VA). .. All cell lines were maintained in Dulbecco's modified essential medium or RPMI 1640 medium supplemented with 10% fetal bovine serum, 10 mM glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin (Life Technologies, Inc., Grand Island, NY).

    Article Title: Design, Synthesis, Analysis, and Cytotoxicity of Novel Heteroaryl Derivatives of Dipyridothiazines.
    Article Snippet: .. Cell Line and Culture The cell lines used in this study—human breast adenocarcinoma (MDA-MB-231), human lung cancer (A-549), and immortal HaCaT keratinocytes—were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA), ensuring their verified origin and adherence to reference characteristics. .. Each line was maintained according to ATCC-recommended specifications for its cell type.

    Article Title: Design and development of 5'-modified 7-substituted 4'-thionucleosides as potent Haspin inhibitors: Synthesis and biological evaluation.
    Article Snippet: Haspin, a serine/threonine kinase essential for proper chromosome alignment during mitosis, has emerged as a promising anticancer target due to its selective function in proliferating cells.. In our previous study, we identified LJ-4827, a 7-acetylene-substituted 4′-thionucleoside analog bearing a 5′-azido group, as a potent Haspin inhibitor with significant antiproliferative effects.. To further optimize activity, we synthesized a series of novel 4′-thionucleoside analogs with 7-substituted scaffolds and diverse 5′-position modifications, as well as selected 6-position derivatives.

    Cell Culture:

    Article Title: 2D van der Waals System Integrated with Targeting Radio-Diagnostic and Imaging Functions.
    Article Snippet: .. Cell Culture: To evaluate the uptake rates of nanoplates based on the FR expression level of various cells, human lung cancer (H460 and A549) and cervical cancer (KB and HeLa) cell lines were purchased from the American Type Culture Collection (ATCC). .. H460, A549, and KB cells were cultured in the RPMI 1640 medium (Corning Inc., Corning, NY, USA) with 10% fetal bovine serum (FBS), 1% antibiotics and antimycotics (100 units mL−1 of penicillin and 100 mg mL−1 of streptomycin; Invitrogen, Thermo Fisher Scientific) under a humidified atmosphere (5% CO2 and 95% air) at 37 °C.

    Expressing:

    Article Title: 2D van der Waals System Integrated with Targeting Radio-Diagnostic and Imaging Functions.
    Article Snippet: .. Cell Culture: To evaluate the uptake rates of nanoplates based on the FR expression level of various cells, human lung cancer (H460 and A549) and cervical cancer (KB and HeLa) cell lines were purchased from the American Type Culture Collection (ATCC). .. H460, A549, and KB cells were cultured in the RPMI 1640 medium (Corning Inc., Corning, NY, USA) with 10% fetal bovine serum (FBS), 1% antibiotics and antimycotics (100 units mL−1 of penicillin and 100 mg mL−1 of streptomycin; Invitrogen, Thermo Fisher Scientific) under a humidified atmosphere (5% CO2 and 95% air) at 37 °C.



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    Internalization of SD-EVLPs and its inhibitory effects on lung cancer cell proliferation (A) Internalization of SD-EVLPs by lung cancer cells; (B, C) CCK-8 assays assessing the effects of SD-EVLPs on lung cancer cell viability; (D–G) EdU assays evaluating the proliferation of <t>A549</t> and NCI-H1299 cells; (H–I) Colony formation assays assessing the proliferative capacity of A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.
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    Internalization of SD-EVLPs and its inhibitory effects on lung cancer cell proliferation (A) Internalization of SD-EVLPs by lung cancer cells; (B, C) CCK-8 assays assessing the effects of SD-EVLPs on lung cancer cell viability; (D–G) EdU assays evaluating the proliferation of <t>A549</t> and NCI-H1299 cells; (H–I) Colony formation assays assessing the proliferative capacity of A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.
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    Internalization of SD-EVLPs and its inhibitory effects on lung cancer cell proliferation (A) Internalization of SD-EVLPs by lung cancer cells; (B, C) CCK-8 assays assessing the effects of SD-EVLPs on lung cancer cell viability; (D–G) EdU assays evaluating the proliferation of A549 and NCI-H1299 cells; (H–I) Colony formation assays assessing the proliferative capacity of A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Journal: Frontiers in Oncology

    Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

    doi: 10.3389/fonc.2026.1829211

    Figure Lengend Snippet: Internalization of SD-EVLPs and its inhibitory effects on lung cancer cell proliferation (A) Internalization of SD-EVLPs by lung cancer cells; (B, C) CCK-8 assays assessing the effects of SD-EVLPs on lung cancer cell viability; (D–G) EdU assays evaluating the proliferation of A549 and NCI-H1299 cells; (H–I) Colony formation assays assessing the proliferative capacity of A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

    Techniques: CCK-8 Assay, Control

    SD-EVLPs inhibit migration and invasion of lung cancer cells (A–D) Wound healing assays assessing the effect of SD-EVLPs on the migration of A549 and NCI-H1299 cells; (E, F) Transwell assays evaluating the invasion ability of A549 and NCI-H1299 cells; (G–I) Western blot analysis of E-cadherin and N-cadherin protein expression. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Journal: Frontiers in Oncology

    Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

    doi: 10.3389/fonc.2026.1829211

    Figure Lengend Snippet: SD-EVLPs inhibit migration and invasion of lung cancer cells (A–D) Wound healing assays assessing the effect of SD-EVLPs on the migration of A549 and NCI-H1299 cells; (E, F) Transwell assays evaluating the invasion ability of A549 and NCI-H1299 cells; (G–I) Western blot analysis of E-cadherin and N-cadherin protein expression. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

    Techniques: Migration, Western Blot, Expressing, Control

    RT-qPCR and Western blot analysis of ferroptosis-related gene expression in lung cancer cells (A, B) RT-qPCR analysis of GPX4 and SLC7A11 mRNA expression in A549 cells; (C, D) RT-qPCR analysis of GPX4 and SLC7A11 mRNA expression in NCI-H1299 cells; (E–H) Western blot analysis of GPX4 and xCT protein expression in lung cancer cells; (I) TEM assessment of mitochondrial ultrastructure. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Journal: Frontiers in Oncology

    Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

    doi: 10.3389/fonc.2026.1829211

    Figure Lengend Snippet: RT-qPCR and Western blot analysis of ferroptosis-related gene expression in lung cancer cells (A, B) RT-qPCR analysis of GPX4 and SLC7A11 mRNA expression in A549 cells; (C, D) RT-qPCR analysis of GPX4 and SLC7A11 mRNA expression in NCI-H1299 cells; (E–H) Western blot analysis of GPX4 and xCT protein expression in lung cancer cells; (I) TEM assessment of mitochondrial ultrastructure. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

    Techniques: Quantitative RT-PCR, Western Blot, Gene Expression, Expressing, Control

    SD-EVLPs modulate the FABP4/PPARG/GPX4-associated pathway in lung cancer cells (A–D) RT-qPCR analysis of FABP4 and PPARG mRNA expression in A549 and NCI-H1299 cells; (E–H) Western blot analysis of FABP4 and PPARG protein expression in A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Journal: Frontiers in Oncology

    Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

    doi: 10.3389/fonc.2026.1829211

    Figure Lengend Snippet: SD-EVLPs modulate the FABP4/PPARG/GPX4-associated pathway in lung cancer cells (A–D) RT-qPCR analysis of FABP4 and PPARG mRNA expression in A549 and NCI-H1299 cells; (E–H) Western blot analysis of FABP4 and PPARG protein expression in A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

    Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Control