human lung cancer 95c cells  (ATCC)

Journal: Frontiers in Oncology

Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

doi: 10.3389/fonc.2026.1829211

Figure Lengend Snippet: Internalization of SD-EVLPs and its inhibitory effects on lung cancer cell proliferation (A) Internalization of SD-EVLPs by lung cancer cells; (B, C) CCK-8 assays assessing the effects of SD-EVLPs on lung cancer cell viability; (D–G) EdU assays evaluating the proliferation of A549 and NCI-H1299 cells; (H–I) Colony formation assays assessing the proliferative capacity of A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

Techniques: CCK-8 Assay, Control

Journal: Frontiers in Oncology

Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

doi: 10.3389/fonc.2026.1829211

Figure Lengend Snippet: SD-EVLPs inhibit migration and invasion of lung cancer cells (A–D) Wound healing assays assessing the effect of SD-EVLPs on the migration of A549 and NCI-H1299 cells; (E, F) Transwell assays evaluating the invasion ability of A549 and NCI-H1299 cells; (G–I) Western blot analysis of E-cadherin and N-cadherin protein expression. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

Techniques: Migration, Western Blot, Expressing, Control

Journal: Frontiers in Oncology

Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

doi: 10.3389/fonc.2026.1829211

Figure Lengend Snippet: RT-qPCR and Western blot analysis of ferroptosis-related gene expression in lung cancer cells (A, B) RT-qPCR analysis of GPX4 and SLC7A11 mRNA expression in A549 cells; (C, D) RT-qPCR analysis of GPX4 and SLC7A11 mRNA expression in NCI-H1299 cells; (E–H) Western blot analysis of GPX4 and xCT protein expression in lung cancer cells; (I) TEM assessment of mitochondrial ultrastructure. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

Techniques: Quantitative RT-PCR, Western Blot, Gene Expression, Expressing, Control

Journal: Frontiers in Oncology

Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

doi: 10.3389/fonc.2026.1829211

Figure Lengend Snippet: SD-EVLPs modulate the FABP4/PPARG/GPX4-associated pathway in lung cancer cells (A–D) RT-qPCR analysis of FABP4 and PPARG mRNA expression in A549 and NCI-H1299 cells; (E–H) Western blot analysis of FABP4 and PPARG protein expression in A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Control

Journal: bioRxiv

Article Title: Scalable longitudinal imaging and transcriptomics of cells in dynamic enclosures

doi: 10.64898/2026.05.05.723030

Figure Lengend Snippet: A: classification of CCEs in different phenotypes based on the analysis of longitudinal imaging data. Red: CellTrace™ Far Red, blue: Annexin V, green: EGFR. B: UMAP based on the transcriptomic data from 10,604 CCEs containing A549 cells treated with 10 µM Olmutinib. The colors represent different transcriptomic clusters. C: UMAP based on the transcriptomic data (same as panel B) colored according to the imaging-derived CCE classification in panel A. 2,328 CCEs that could not be accurately classified were excluded from the analysis. D: proportion of CCEs (y axis) belonging to each imaging-based phenotype (indicated by the color) within each gene expression cluster (x axis). E: Upset plot showing overlap of significant GSEA pathway enrichments across three classification strategies. The combination of transcriptomic clustering with imaging classification identified 15 unique pathways not found in either single-modality strategy. F: significant interaction effects (p_adj < 0.05) between RNA clusters and imaging phenotypes on the prediction of drug resistance pathway modules (G2M checkpoint, E2F targets, MYC targets, DNA repair, EMT) (see Methods). The daughter cell resistant phenotype showed 7 out of 14 total significant interactions, indicating that pathway activities are maximally explained by the combination of transcript state and the daughter cell resistant phenotypic classification. G: Confusion matrix for elastic net prediction of imaging phenotypes from gene expression. H: STRING PPI network for top 50 positive coefficient genes (associated with daughter cell resistance). I: STRING PPI network for top 50 negative coefficient genes (inversely associated with daughter cell resistance). J: Selected differentially expressed genes between expression-defined clusters (x axis). The color represents the average expression (scaled per gene) and the size of the circle indicates the percentage of CCEs expressing the gene. Cluster 2 showed strong enrichment for cell division pathways and overexpressed the proliferation marker TOP2A. Cluster 3 exhibited activation of multiple EGFR bypass pathways with overexpression of EPHA7 (64), HGF (65), ERBB2 (66), and AXL(67), all capable of activating MAPK signaling independently of EGFR. Cluster 5 displayed enrichment of p53 targets, including upregulation of quiescence-associated genes such as GADD45A, REDD1, ATF3, SFN, and BTG2.

Article Snippet: Human lung cancer A549 cells were purchased from ATCC (CCL-185), and cultured in DMEM supplemented with 10% FBS and 1% Pen-Strep.

Techniques: Imaging, Derivative Assay, Gene Expression, Expressing, Marker, Activation Assay, Over Expression